Anatomical Model
Oxyhemoglobin (O₂Hb)
Highly oxygenated blood entering the tissue. In near-infrared spectroscopy, 830 nm light is primarily absorbed by O₂Hb because of its specific chemical properties and oxygenated state.
Level
Deoxyhemoglobin (HHb)
Deoxygenated blood returning through capillary networks. 690 nm light exhibits higher optical absorption for HHb, making it the primary target wavelength for detecting oxygen depletion.
Level
fNIRS Signal Display
Concentration Changes (ΔO₂Hb, ΔHHb, Δ[HbT]) over time
Optical Absorption Spectrum
Extinction coefficient of O₂Hb and HHb across NIR window
Note the crossover point (isosbestic point) at approximately 800 nm, where extinction coefficients for both oxygenation states are equal.
Interactive Science Guide
Neurovascular Coupling
Clicking "Trigger Neural Activation" stimulates neurons. This triggers localized oxygen consumption and triggers a rapid increase of regional cerebral blood flow.
The Near-Infrared Window
Living tissues are transparent to NIR light (650-900nm), allowing photons to penetrate scalp, skull, and CSF, reaching up to 1-2cm into cerebral cortex.
Modified Beer-Lambert Law
Because light scatters in a banana-shaped path, the modified Beer-Lambert Law calculates relative concentration changes of O₂Hb and HHb based on optical density changes.